Effect of Dihydroartemisinin on contractility to Drugs in isolated smooth muscle Preparations in guinea pig in vitro models

This study was aimed at evaluating the effect of Dihydroartemisinin (DHA)on contractility to drugs in ileum isolated smooth muscle preparations guinea pigs using Vitro experimental procedures / standard protocols an organ bath set up. dihydroartemisinin (1.0 x 10 - 8 1.0 – 5mg/mL) when applied alone and separately excited marked variable effects on pig . In some preparation it showed no response, while others produced slight phasic contraction external calcium (Ca2+) ion introduced. The contractile activity abolished by verapamil (5x10-3mg/mL). 6 caused significant induced dependent inhibition acetylcholine, potassium chloride histamine depolarizing tyrode solution (p < 0.05).The Inhibitory response maxima acetylcholine is 17.0±0.1 mm(65.56% inhibition) ; moderate compared inhibitory atropine (10-5 M). result shows that seems act via non-specific receptor mechanism, with appreciable channel blocking safe therapeutic doses.